GHK-Cu — In Vitro Research Overview

GHK-Cu is a copper-tripeptide research compound used by researchers in extracellular matrix remodeling, fibroblast assays, and copper-chaperone biology.

Chemical Identity

In Vitro Research Context

GHK-Cu (Glycyl-L-histidyl-L-lysine copper complex, CAS 49557-75-7) is a naturally occurring tripeptide-copper complex studied extensively in vitro. Research applications center on its effects in dermal fibroblast assays, extracellular matrix (ECM) synthesis, and metalloproteinase regulation. This batch-verified research compound is used by laboratory researchers studying the role of copper signaling peptides in cell biology.

In vitro studies have characterized GHK-Cu interactions with integrins, collagen synthesis pathways, and the regulation of matrix metalloproteinases (MMPs). Researchers in wound-healing cell models and dermatological in vitro platforms use this research peptide to study how copper-tripeptide complexes modulate fibroblast behavior and ECM composition. All uses described are in vitro; supplied for Research Use Only (RUO).

Research Applications

The following in vitro laboratory research applications have been reported in published literature. All listed uses are strictly in vitro — this research compound is not for in vivo, human, or veterinary use.

• Fibroblast proliferation and migration assays
• Collagen synthesis in vitro studies (collagen I, III, IV)
• Matrix metalloproteinase (MMP-1, MMP-2) regulation research
• Copper-chaperone signaling research in cell culture
• Dermal ECM remodeling models
• Anti-oxidant enzyme induction studies in cell lines

Laboratory Notes

Lyophilized powder is stable at −20°C for 24 months in sealed original packaging. Protect from direct light.

Batch Verification

Every batch of this research compound is independently tested by Freedom Diagnostics, an ISO 17025 accredited independent analytical laboratory. Testing includes:

• HPLC-UV purity determination (reported as % area)
• LC-MS sequence and molecular weight confirmation
• Net content (mg) gravimetric verification
• Full Certificate of Analysis (COA) with batch number, accession number, and laboratory stamp

The COA batch number is printed on every vial and scannable via QR code for direct verification. Researchers can cross-reference the accession number against the Freedom Diagnostics laboratory record independently.

External Research References

The following resources provide background context for researchers studying this compound:

• GHK-Cu on Wikipedia
• Extracellular matrix biology
• In vitro research methodology
• HPLC purity testing
• ISO 17025 accreditation standard